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Product Info Summary
| SKU: | A00590 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Clusterin Antibody
View all Clusterin/APOJ Antibodies
SKU/Catalog Number
A00590
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Clusterin Antibody (Catalog # A00590). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Clusterin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Clusterin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00590)
Host
Rabbit
Contents
Clusterin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Clusterin antibody was raised recombinant human Clusterin isoform 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.
Reactive Species
A00590 is reactive to CLU in Human, Mouse
Applications
A00590 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.495kDa
Background of Clusterin/APOJ
Clusterin, also known as Apolipoprotein J (ApoJ), is a ubiquitous multifunctional glycoprotein that can interact with a broad spectrum of molecules such as complement components, various receptors, and the Alzheimer's b-amyloid peptide. Clusterin expression is increased in Alzheimer's disease brain tissue and clusterin-immunoreactive amyloid plaques are found associated with phospho-tau-positive dystrophic neurites and it has been suggested that clusterin facilitates the conversion of diffuse b-amyloid deposits into amyloid and enhances tau phosphorylation in neurites around these plaques. Other reports show that clusterin expression is decreased in proliferating cells and is upregulated in quiescent and senescent cells, suggesting that it may also play a role in aging and tumorigenesis suppression. Clusterin exists in at least two distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Clusterin antibody can be used for the detection of Clusterin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Clusterin in human brain tissue lysate with Clusterin body at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of Clusterin in human brain tissue with Clusterin antibody at 5 μg/mL.
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Immunofluorescence of Clusterin in human brain tissue with Clusterin antibody at 20 μg/ml.
Red: Clusterin Antibody (A00590)
Blue: DAPI staining
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Immunohistochemistry of Clusterin in human brain tissue with Clusterin antibody at 10 μg/mL.
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Immunohistochemistry of Clusterin in mouse brain tissue with Clusterin Antibody at 5 μg/mL.
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Immunofluorescence of Clusterin in mouse brain tissue with Clusterin Antibody at 20 μg/mL.
Green: Clusterin Antibody (A00590)
Red: Phylloidin staining
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For CLU (Source: Uniprot.org, NCBI)
Gene Name
CLU
Full Name
Clusterin
Weight
52.495kDa
Superfamily
clusterin family
Alternative Names
40; 40, sulfated glycoprotein 2; Aging-associated gene 4 protein; aging-associated protein 4; APOJ; apo-J; Apolipoprotein J; CLI; CLIclusterin (complement lysis inhibitor, SP-40; CLU; Clusterin; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40; Ku70-binding protein 1; KUB1SGP2; MGC24903; NA1/NA2; SGP-2; SP-40; sulfated glycoprotein 2; Testosterone-repressed prostate message 2; testosterone-repressed prostate message 2, apolipoprotein J); TRPM-2; TRPM-2TRPM2 CLU AAG4, APO-J, APOJ, CLI1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, CLU clusterin clusterin|aging-associated protein 4|apolipoprotein J|complement cytolysis inhibitor|complement lysis inhibitor|complement-associated protein SP-40,40|epididymis secretory sperm binding protein|ku70-binding protein 1|sulfated glycoprotein 2|testosterone-repressed prostate message 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLU, check out the CLU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Clusterin Antibody (A00590)
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No publications found for A00590
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